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. 2014 Jul 14;9(7):e102582. doi: 10.1371/journal.pone.0102582

Figure 4. Rb regulates mitochondrial membrane proteins expression.

Figure 4

(A) mRNA and protein levels of Bax, Bak and Bad in 3T3/wt and 3T3/Rb−/− cells were determined by RT-PCR and western blot. The number indicates quantified ratio relative to the inter-control GAPDH or β-Actin. (B) Bad protein levels in 3T3/Rb−/− cells with or without Rb re-expression. The number indicates quantified ratio relative to the inter-control β-Actin. (C) Bcl-XL was over-expressed in 3T3/Rb−/− cells. (D) FL2/FL1 ratios were determined based on JC-1 fluorescence after 3T3/Rb−/− cells with or without Bcl-XL over-expression were cultured for 24 h. (E) ROS levels were determined based on DCF fluorescence after 3T3/Rb−/− cells with or without Bcl-XL over-expression were cultured for 24 h. *P<0.05, **P<0.01.