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Immunohistochemistry expression pattern of GRB2 in ESCC. Negative immunoreactivity was observed when GRB2 antibody was replaced with PBS. The representative immunohistochemistry visual fields in this image were scored as follows: Immunoreactive scores for GRB2 was 4 in the cytoplasm of noncancerous tissues, 4 in cancer cytoplasm of low-expression group and 12 in cancer cytoplasm of overexpression group. The inserted bars are 50 μm.
