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. 2014 Jul 14;9(7):e102590. doi: 10.1371/journal.pone.0102590

Figure 3. Effect of EpCAM silencing and/or 5-FU treatment on apoptosis in MCF-7 cells.

Figure 3

A: Apoptosis was examined using annexin V-FITC/PI staining and flow cytometry analysis. A representative flow cytometric analysis of apoptosis in MCF-7 cells is shown. The fluorescence intensity of annexinV/FITC is plotted on the x-axis, and PI is plotted on the y-axis. FITC−/PI−, FITC+/PI−, FITC+/PI+, FITC−/PI+ was regarded as living, early apoptotic, late apoptotic and necrotic cells, respectively. B: The percentage of apoptotic cells was examined by annexin V-FITC/PI staining and flow cytometry analysis. Results are presented as mean±SD of three separate experiments. *P<0.05 versus 5-FU.