Figure 5. Effect of si-EpCAM and/or 5-FU treatment on apoptosis-related factors in MCF-7 cells.

(A) MCF-7 cells were treated with 7.5 µg/ml and 20 µg/ml 5-FU for 48 h. Cells were harvested and analyzed by western blotting with antibodies against Bcl-2, Bax and caspase3. (B) MCF-7 cells were treated with si-EpCAM and/or 5-FU (7.5 µg/ml) for 48 h, and the expression of Bcl-2, Bax and caspase 3 was determined by immunoblotting.