Figure 6. Involvement of the ERK1/2 and JNK signaling pathways in si-EpCAM and 5-FU induced apoptosis.

(A) Effect of si-EpCAM and/or 5-FU treatment on the ERK and JNK signalingpathways.MCF-7 cells were treated with si-EpCAM and/or 5-FU (7.5 µg/ml) for 48 h, and the expression of pJNK and pERK1/2 was determined by immunoblotting. (B) The schematic illustration summarizes the effect of si-EpCAM on apoptosis in MCF-7 cells through activation of ERK and JNK signaling pathways induced by 5-FU.