FIG 2.

BRSV protein content in BRSV-ISCOMs, BRSV proteins, and Vero-ISCOMs. (a) Coomassie blue-stained SDS-PAGE gel showing the bands analyzed by mass spectrometry and tandem mass spectrometry; (b) dot blots using monoclonal antibodies against BRSV proteins; (c) Western blots using monoclonal antibodies against HRSV F and BRSV G and M, rabbit polyclonal antiserum against recombinant HRSV M2-1, N, and P, and serum from an unvaccinated experimentally BRSV-infected calf. Arrows indicate BRSV proteins.