TABLE 1.
MALDI-TOF MS analysis of proteins present in the BRSV-ISCOMs
| Molecular mass (kDa)a | BRSV-ISCOM (aa)b |
BRSV protein (aa)b |
Vero-ISCOMb |
||
|---|---|---|---|---|---|
| INRA | UU | INRA | UU | UU | |
| 120 | Integrin α3c | ||||
| 117 | Integrin αVc | Integrin αVc | |||
| 112 | Integrin αVc | ||||
| 93 | Integrin α3c,e | Integrin α3c | |||
| 92 | Integrin β1c | Integrin β1c | |||
| 90 | Integrin β3c | ||||
| 84 | Integrin β3c | Integrin α3c | Integrin Aiibb3c | ||
| 72 | 78-kDa glucose-related proteinc,e | ||||
| 64 | BRSV F protein (207–520, 157–336) | BRSV F protein (157–461)e | BRSV F protein (157–520) | BRSV F protein (157–520) | |
| 54 | BRSV F protein (62–331) | ||||
| 44 | BRSV N protein (8–308) | BRSV N protein (7–352) | BRSV N protein (47–358) | BRSV N protein (7–520) | |
| 31 | Porin 31 HM | ||||
| NAf | BRSV F protein/CD9c,g | BRSV F protein/CD9g | |||
| 11 | Histone H4 type VIIId | Histone H4 type VIIId | |||
a
Protein molecular mass as estimated by mass spectrometry.
b
Amino acid sequences (aa) covering the region of matched protein are in parentheses. UU, Uppsala University.
c
Matches an amino acid sequence of protein originating from Homo sapiens or Macaca mulatta (proteins probably originating from Vero cells).
d
Matches an amino acid sequence of protein originating from Gallus gallus (histones are almost identical between species).
e
Nonsignificant match (score is between 55 and 70) (P = 0.05).
f
NA, not analyzed.
g
Results were obtained by tandem mass spectrometry.