TABLE 4.
Growth rates for salt-dependent PstS, PstC, and PstA PBr mutants
| Salts | Growth ratea |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | PstS mutantb: |
PstC mutant 3.2b | PstA mutant 10.9b | |||||||||
| Δ | 1.1 | 2.1 | 3.1 | 3.3 | 3.7 | 4.4 | 4.5 | 10.14 | ||||
| Nonec | 46.1 | ND | 99.4 | 127.0 | 173.1 | 133.0 | 158.8 | 104.0 | 186.3 | 177.6 | 200.3 | ND |
| K2HPO4, MgSO4, NaCl | 51.9 | 58.7 | 54.0 | 55.6 | 58.2 | 55.5 | 52.9 | 52.5 | 65.3 | 56.8 | 59.7 | 59.0 |
a
Growth rate (t1/2) was measured in salt-reduced C medium supplemented with indicated salts.
b
PBr mutants containing mutations within pstS, pstC, and pstA are described in Table 1 and in Table S6 in the supplemental material. ND, none detected.
c
Final yields for PBr mutants containing SNPs in pstS or pstC reached approximately 60% of the WT value after 24 h of culture in the absence of salts and 100% in the presence of salts.