TABLE 5.

Auxotrophic P_i revertants recovered following 48 h of culture in salt-reduced C medium

Mutanta	Growthb	Ratec
PstS
Δ	0/10	ND
1.1	10/10	46.3
2.1	10/10	49.0
3.1	10/10	45.3
3.3	10/10	48.0
3.7	10/10	45.7
4.4	10/10	53.8
4.5	10/10	48.7
10.14	10/10	48.6
PstC mutant 3.2	1/10	46.3
PstA mutant 10.9	1/10	50.2

^aPBr mutants containing mutations within pstS, pstC, and pstA are described in Table 1 and in Table S6 in the supplemental material. Pst revertant mutants are described in detail in Table S2.

^bPBr mutants were subcultured 1:1,000 from C medium into salt-reduced C medium. Ability to grow in salt-reduced C medium was scored in each of 10 replicate culture tubes after 24 h by measurement of OD₆₀₀.

^cGrowth rate (t_1/2) of revertant mutants was measured in salt-reduced C medium. ND, none detected.