TABLE 3.
Quantification of aggregate numbers and cell lengths in microscopy fields captured after 4 h of coexpression of the specified RepA-WH1 pairsa
| RepA-WH1 pair | % of cells with the indicated no. of aggregates |
Avg cell length (μm) | ||||
|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | ≥4 | ||
| WT-mRFP + WT-YFP | 77.5 | 16 | 6.5 | 0 | 0 | 2.67 ± 0.79 |
| WT-mRFP + A31V-YFP | 0 | 7 | 58.5 | 19.5 | 15.5 | 5.07 ± 1.46 |
| WT-mRFP + YFP | 100 | 0 | 0 | 0 | 0 | 3.87 ± 1.13 |
| WT-mRFP + ΔN37-YFP | 0 | 43 | 39 | 13.5 | 4.5 | 3.91 ± 1.30 |
| A31V-mRFP + WT-YFP | 0 | 5.5 | 41.5 | 33.5 | 19.5 | 3.89 ± 1.32 |
| A31V-mRFP + A31V-YFP | 0 | 5 | 56 | 20.5 | 18.5 | 5.00 ± 1.66 |
| A31V-mRFP + YFP | 11 | 6 | 37.5 | 17.5 | 28 | 5.25 ± 1.54 |
| A31V-mRFP + ΔN37-YFP | 0 | 9 | 39.5 | 27 | 24.5 | 5.27 ± 1.58 |
| mRFP + WT-YFP | 100 | 0 | 0 | 0 | 0 | 2.33 ± 0.49 |
| mRFP + A31V-YFP | 0 | 10 | 64 | 21 | 5 | 4.91 ± 1.26 |
| mRFP + YFP | 100 | 0 | 0 | 0 | 0 | 4.23 ± 1.24 |
| mRFP + ΔN37-YFP | 0 | 90 | 10 | 0 | 0 | 3.21 ± 0.83 |
| ΔN37-mRFP + WT-YFP | 45 | 52.5 | 2.5 | 0 | 0 | 2.04 ± 0.59 |
| ΔN37-mRFP + A31V-YFP | 0 | 5.5 | 63.5 | 18.5 | 12.5 | 5.00 ± 1.49 |
| ΔN37-mRFP + YFP | 86.5 | 11.5 | 2 | 0 | 0 | 3.79 ± 1.15 |
| ΔN37-mRFP + ΔN37-YFP | 0 | 68 | 27.5 | 3 | 1.5 | 3.33 ± 0.99 |
a
A total of 200 cells of each class were used for these measurements.