TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Sinorhizobium meliloti | ||
| Rm1021 | Wild-type strain (Smr) | 46 |
| GMI11495 | Wild-type strain (Smr), Rm2011 background | 9, 47 |
| 2011mTn5STM.2.04.C11 | GMI11495 SMb20515::mTn5 | 47 |
| 2011mTn5STM.3.10.F07 | GMI11495 SMa0113::mTn5 | 47 |
| 2011mTn5STM.3.08.E09 | GMI11495 SMb20933::mTn5 | 47 |
| 2011mTn5STM.4.06.A07 | GMI11495 SMa1696::mTn5 | 47 |
| CBT208 | Rm1021 rpoE2::hph (Hygr) | 5 |
| CBT430 | Rm1021 ΔrsiB1 ΔrsiB2 | 23 |
| CBT785 | Rm1021 ΔrsiC | This work |
| CBT862 | Rm1021 ΔrsiC ΔSMa1001 | This work |
| CBT866 | Rm1021 ΔrsiC ΔSMa2063 | This work |
| CBT1051 | Rm1021 ΔrsiC ΔrsiB1 ΔrsiB2 | This work |
| CBT1129 | Rm1021 ΔSMc00322 | This work |
| CBT1169 | Rm1021 ΔrsiC ΔSMc00322 | This work |
| CBT1702 | GMI11495 ΔrsiC SMa0113::mTn5 | This work |
| CBT1704 | GMI11495 ΔrsiC SMb20515::mTn5 | This work |
| CBT1706 | GMI11495 ΔrsiC SMa1696::mTn5 | This work |
| CBT1708 | GMI11495 ΔrsiC SMb20933::mTn5 | This work |
| CBT1710 | GMI11495 ΔrsiC | This work |
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169 Φ80dlacZ ΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| Plasmids | ||
| pGEM-T | Cloning vector (Ampr) | Promega |
| pJQ200mp19 | Gene replacement vector (Gmr) | 48 |
| pRK2013 | Helper plasmid for triparental matings (Kanr) | 49 |
| pMP220-885 | pMP220-PSMc00885-lacZ fusion (Tetr) | 23 |
| pMLBAD | Expression vector, inducible by arabinose (Tmpr) | 50 |
| pMLBAD-rsiB1 | pMLBAD derivative expressing rsiB1 | 23 |
| pMLBAD-rsiB2 | pMLBAD derivative expressing rsiB2 | 23 |
| pMLBAD-rsiB1-D191A | pMLBAD derivative expressing rsiB1-D191A | 23 |
| pMLBAD-rsiB2-D191A | pMLBAD derivative expressing rsiB2-D191A | This work |
| pMLBAD-rsiB1-strep | pMLBAD derivative expressing rsiB1-strep | This work |
| pMLBAD-rsiC | pMLBAD derivative expressing rsiC | This work |
| pMLBAD-rsiC-H318K | pMLBAD derivative expressing rsiC-H318K | This work |
| pLS100-17 | pJQ200mp19 derivative for rsiC deletion | This work |
| pLS166-5 | pJQ200mp19 derivative for SMc00322 deletion | This work |
| pLS124-1 | pJQ200mp19 derivative for SMa1001 deletion | This work |
| pLS125-1 | pJQ200mp19 derivative for SMa2063 deletion | This work |