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. 2014 Jul;196(14):2638–2645. doi: 10.1128/JB.01537-14

FIG 1.

FIG 1

ΔsigKLM deletion does not phenocopy Δrip1 in mouse infection. (A) Confirmation of the M. tuberculosis ΔsigKLM strain. Southern blot analysis of M. tuberculosis chromosomal DNA was used to confirm the genotype of a ΔsigKLM triple mutant. When probed with the 3′ flanking region of sigM, EcoRI-digested genomic DNA produces a 12.2-kb band in ΔsigL (ΔL) cells, whereas the ΔsigLM (ΔLM) strain has a 5.2-kb band representing the sigM deletion. When probed with the 5′ flanking region of sigK, PstI-digested genomic DNA produces a 4.8-kb band in the ΔLM strain, indicating a wild-type sigK allele, whereas the ΔsigKLM (ΔKLM) triple mutant strain has a 551-bp band, representing the ΔsigK allele. (B) Phenotype of the Δrip1 strain in mice. Recovered CFU from lungs of mice infected via aerosol with wild-type M. tuberculosis and the Δrip1 strain are depicted. Error bars represent standard deviations and when not visible are within the symbol. (C) Recovered CFU from lungs of mice infected via aerosol with wild-type M. tuberculosis and the ΔsigKLM mutant. (D) Recovered CFU from spleens of mice infected via aerosol with wild-type M. tuberculosis and the ΔsigKLM mutant.