TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype/details | Reference or source |
|---|---|---|
| E. coli strains | ||
| KL16 | E. coli K-12; thi1 relA1 spoT1 | 18 |
| KL16ΔmetY::cm | Derivative of KL16 with the metY gene replaced with a 1.1-kb Cmr cassette | 16 |
| KL16ΔmetZWV::kan | Derivative of KL16 with the metZWV genes replaced with a 1.4-kb Kanr cassette | 16 |
| CA274 | E. coli K-12; hfr lacZ125am trpA49am relA1 spoT1 | 19 |
| Su15 | Derivative of CA274; metYCUA | 14 |
| CP78 | E. coli K-12; relA+ spoT+; mutated in leu, arg, his, thr thi, relX | 20 |
| CP78(Tet) | Derivative of CP78; Tetr; ∼70% linkage with metY | Laboratory strain, unpublished |
| CP78(Tet)Su15 | Derivative of CP78; Tetr; ∼70% linkage with metYCUA | Laboratory strain, unpublished |
| CP78ΔmetY::kan | Derivative of CP78 with the metY gene replaced with a 1.4-kb Kanr cassette | This study |
| CP79 | E. coli K-12; spoT+; mutated in leu, arg, his, thr, thi, relA, relX | 20 |
| CP79(Tet) | Derivative of CP79; Tetr | This study |
| CP79(Tet)Su15 | Derivative of CP79; Tetr; ∼70% linkage with metYCUA | This study |
| MG1655 | ilvG rfb-50 rph-1 | 21 |
| MG(Tet) | Derivative of MG1655; Tetr; ∼70% linkage with metY | This study |
| MG(Tet)Su15 | Derivative of MG1655; Tetr; ∼70% linkage with metYCUA | This study |
| MGΔmetY::kan | Derivative of MG1655 with the metY gene replaced with a 1.4-kb Kanr cassette | This study |
| Plasmids | ||
| pCATam1 | Renamed from pRSVCATam1.2.5; pBR322 derivative harboring the CAT reporter gene with UAG as the initiation codon; Ampr | 22 |
| pmetY | Derivative of pCATam1 plasmid encoding the wild-type metY gene and lacking the CAT reporter gene | 16 |