Characterization of the ΔAlgO mutant. (A) Growth profile of strain A1 WT and ΔAlgO mutant cells in alginate, yeast extract, glucose, or glucuronic acid medium. Closed circles, WT; open circles, ΔAlgO mutant. Growth experiments were performed in triplicate. Error bars represent the standard deviations of the means of the three experiments. (B) Western blotting. Left, WT cell extracts from each medium; right, ΔAlgO mutant cell extracts from each medium. Lane 1, alginate; lane 2, yeast extract; lane 3, glucose; lane 4, glucuronic acid.