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. 2014 Jul;196(14):2691–2700. doi: 10.1128/JB.01666-14

FIG 5.

FIG 5

Gel mobility shift assay. (A) Alginate genetic cluster. Probe 1, algO-algS intergenic region; probe 2, algQ1-algQ2 intergenic region; probe 3, algQ2–a1-IV intergenic region. (B) Gel mobility shift assay with DIG-labeled DNA probes and AlgO-His6. Lane 1, probe 1; lane 2, probe 1 and AlgO-His6; lane 3, probe 1, nonlabeled probe 1, and AlgO-His6; lane 4, negative control DIG-labeled probe and AlgO-His6; lane 5, probe 2; lane 6, probe 2 and AlgO-His6; lane 7, probe 3; lane 8, probe 3 and AlgO-His6. (C) Probes 4 to 8 in the algO-algS intergenic region. (D) Gel mobility shift assay with probes 4 to 8. Symbols: −, each probe in the absence of AlgO-His6; +, each probe in the presence of AlgO-His6. The closed and open arrowheads indicate the positions of the DNA-protein complex and free DNA, respectively.