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. 2014 Jul;196(14):2691–2700. doi: 10.1128/JB.01666-14

FIG 7.

FIG 7

Effects of alginate derivatives on AlgO binding to DNA. (A) Gel mobility shift assay with DIG-labeled probe 1 in the presence of each alginate derivative. Lane 1, probe 1; lane 2, probe 1 and AlgO-His6; lanes 3 to 10, probe 1 and AlgO-His6 in the presence of 25 mM Δ3G (lane 3), Δ4G (lane 4), Δ5G (lane 5), Δ3M (lane 6), Δ4M (lane 7), chitotriose (lane 8), and chitotetraose (lane 9). (B) Concentration dependence of the inhibitory effect of AlgO binding to DIG-labeled probe 1. Lanes 1 to 4, probe 1 and AlgO-His6 in the presence of 0 mM (lane 1), 10 mM (lane 2), 25 mM (lane 3), and 50 mM (lane 4) of each oligosaccharide. The closed and open arrowheads indicate the positions of the DNA-protein complex and free DNA, respectively.