(A to C) Growth (OD565) and magnetic response (Cmag) of MSR-1 WT, Δcbb3, and Δaa3 Δcbb3 strains under different conditions. (A) Aerobic conditions in nitrate medium; (B) aerobic conditions in ammonium medium; (C) microaerobic conditions in ammonium medium. (D) TEM images of microaerobically grown WT (i), Δcbb3 mutant (ii), and Δaa3 Δcbb3 mutant (iii) strains in ammonium medium. Bars, 500 nm (whole cells) and 100 nm (magnetosomes). (E) Measurements of crystal sizes for MSR-1 WT, Δcbb3, and Δaa3 Δcbb3 strains at different time points in microaerobic ammonium medium. Results from representative experiments were determined in triplicate, and values are given as means and standard deviations.