FIG 4.

Zur is required for growth at high levels of Zn. (A) Wild-type (WT) and Δzur::Km bacteria were grown in the presence of concentrations of ZnCl₂ increasing from 0 to 500 μM. The graph depicts percent growth of WT plus vector, the strain with Δzur::Km plus vector, and the strain with Δzur::Km plus pzurMyc relative to untreated (LB only) cells from an 8-h time point. (B) The WT plus vector, the Δzur::Km strain plus vector, and the Δzur::Km strain plus pzurMyc were grown in the presence of 400 μM ZnCl₂. The graph shows OD₆₀₀ over time up to 24 h and depicts data from three separate experiments. Statistical significance was determined by two-way ANOVA. *, P ≤ 0.0005.