FIG 5.

Zur is required for growth in Zn-limiting conditions. (A) WT plus vector, the Δzur::Km strain plus vector, and the Δzur::Km strain plus pzurMyc grown in the presence of 20 μM TPEN. The graph depicts percent growth of TPEN-treated WT plus vector, Δzur::Km plus vector, and Δzur::Km plus pzurMyc cells over untreated (LB only) cells from an 8-h time point. (B) WT plus vector, the Δzur::Km strain plus vector, and the Δzur::Km strain plus pzurMyc grown in the presence of 20 μM TPEN. The graph shows the OD₆₀₀ of each strain over time up to 10 h. Data presented are consolidated from three separate experiments. Statistical significance was determined by Student's t test. *, P ≤ 0.005.