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. 2014 Jul;196(14):2514–2525. doi: 10.1128/JB.01575-14

FIG 5.

FIG 5

The accumulation of (p)ppGpp increases the proportion of cells with one chromosome. (A) Histograms of the DNA content of cells accumulating (p)ppGpp. Representative profiles were obtained by flow cytometry analysis of unsynchronized cells from strain JC820 expressing RelA′-FLAG upon xylose addition. Cells were grown to exponential phase in PYEG before 0.3% xylose (PYEGX) was added at time zero. Cells were fixed at the indicated times and stained prior to flow cytometry analysis. The horizontal axis indicates the fluorescence intensity of individual cells and the number (N) of complete chromosomes. The vertical axis indicates the number of cells. (B) The proportion of cells with one chromosome increases when (p)ppGpp accumulates. Quantification of the results of flow cytometry experiments using strain JC820 and the control strain JC835. Cells were grown to exponential phase in PYEG before 0.3% xylose (PYEGX) was added to half of the culture at time zero. The y axis corresponds to the average proportion of cells containing 1N chromosome per cell at the indicated times after xylose addition. Plotted values are the averages of three independent populations; the standard deviations are also indicated.