TABLE 1.
Strains and plasmids used in this study
| Plasmid or strain | Relevant characteristics, construction, or genotype | Source or reference |
|---|---|---|
| Plasmids | ||
| pXTCYC-4 | Nonreplicating plasmid for expression of genes under the control of the xylX promoter after integration at the xylX locus in the C. crescentus genome | 61 |
| pXTCYC-4-relA′-FLAG | relA′-FLAG under the control of the xylX promoter in pXTCYC-4 | This study |
| pXTCYC-4-relA′(E335Q)-FLAG | relA′(E335Q)-FLAG under the control of the xylX promoter in pXTCYC-4 | This study |
| pDnaN-RFP | dnaN-mcherry under the control of the native dnaN promoter once integrated into the chromosome | 47 |
| Strains | ||
| E. coli | ||
| TOP10 | Cloning strain | Invitrogen |
| MG1655 | Wild-type strain | 62 |
| C. crescentus | ||
| NA1000 | Synchronizable derivative of wild-type strain CB15 (CB15N) | 63 |
| JC388 | NA1000 dnaN::dnaN-RFP | 47 |
| TFLRa | NA1000 pleC::pleC-YFP divJ::divJ-RFP cpaE::cpaE-CFP | 42 |
| JC835 | NA1000 pXTCYC-4 integrated at the xylX chromosomal locus | This study |
| JC820 | NA1000 pXTCYC-4-relA′-FLAG integrated at the xylX chromosomal locus | This study |
| JC1198 | NA1000 pXTCYC-4-relA′(E335Q)-FLAG integrated at the xylX chromosomal locus | This study |
| JC845 | TFLR pXTCYC-4-relA′-FLAG integrated at the xylX chromosomal locus | This study |
| JC861 | JC388 pXTCYC-4-relA′-FLAG integrated at the xylX chromosomal locus | This study |
a
TLFR, triply fluorescently labeled reporter.