TABLE 1.
Primers used in this study
| Primera | Sequence (5′–3′)b |
|---|---|
| HupB-F | CACTGGATCCGGAGGGTTGGGATGAACAAAGCA (BamHI) |
| HupB-R | GCAAAGGGAATTCGTGTGTCTTGACCTATTTGC (EcoRI) |
| N-HupB-F | CACTGGATCCGGAGGGTTGGGATGAACAAAGCA (BamHI) |
| N-HupB-R | TTCTTGAATTCTCAGGCCCCCACACCACGCTT (EcoRI) |
| N-HupB-R55E-F | GTTCGAACAGCGTCGCGAGGCGGCTCGAGTGGCCC |
| N-HupB-R55E-R | GGGCCACTCGAGCCGCCTCGCGACGCTGTTCGAAC |
| N-HupB-R55Q-F | GTTCGAACAGCGTCGCCAGGCGGCTCGAGTGGCCC |
| N-HupB-R55Q-R | GGGCCACTCGAGCCGCCTGGCGACGCTGTTCGAAC |
| N-HupB-T43A-F | CAAAGGCGACAGCGTCGCCATTACCGGGTTCGGTG |
| N-HupB-T43A-R | CACCGAACCCGGTAATGGCGACGCTGTCGCCTTTG |
| N-HupB-T45A-F | CGACAGCGTCACCATTGCCGGGTTCGGTGTGTTCG |
| N-HupB-T45A-R | CGAACACACCGAACCCGGCAATGGTGACGCTGTCG |
| N-HupB-T65A-F | GCCCGCAATCCGCGTGCCGGCGAGACAGTAAAG |
| N-HupB-T65A-R | CTTTACTGTCTCGCCGGCACGCGGATTGCGGGC |
| N-HupB-T68A-F | CCGCGTACCGGCGAGGCAGTAAAGGTGAAGCCG |
| N-HupB-T68A-R | CGGCTTCACCTTTACTGCCTCGCCGGTACGCGG |
| N-HupB-T74A-F | GTAAAGGTGAAGCCGGCGTCGGTGCCGGCGTTC |
| N-HupB-T74A-R | GAACGCCGGCACCGACGCCGGCTTCACCTTTAC |
| C-HupB-S163A-F | GCTGTCAAGGCCACGAAGGCACCCGCCAAGAAGGTG |
| C-HupB-S163A-R | CACCTTCTTGGCGGGTGCCTTCGTGGCCTTGACAGC |
| PknEK45-M-F | GCGGATCGTGGCACTAATGCTGATGTCGGAGACGC |
| PknEK45-M-R | GCGTCTCCGACATCAGCATTAGTGCCACGATCCGC |
| PknIcat-F | GCGGGCCGTGCCGAATTCGGTTACTATCGGCCA (EcoRI) |
| PknIcat-R | TAGCAACACCTCGAGCGCACCGACCTAGATCCGGCG (XhoI) |
| Rv0884cPromoter region-F | TCGAAGCCTCCCCACCGAGGTGTG |
| Rv0884cPromoter region-R | GCCATCAGGGTAGTGAGGGGTACC |
| Rv1230cPromoter region-F | TTACCGAGTCGGCCATTGCGCCAC |
| Rv1230cPromoter region-R | TCGACCGTCCTCAGTGTGAGCC |
| PknE-F-RT | AACATTCTGGTTAGCGCGGA |
| PknE-R-RT | GCCATGTAGTAGAGGGTGCC |
| PknF-F-RT | TGTGGCATCCACACATCGTC |
| PknF-R-RT | GAAGGGATACGGTGTCGGTG |
| PknB-F-RT | GGTGATGGATTTCGGCATCG |
| PknB-R-RT | CTGTTCGGGTGACAGGTACT |
| HupB-F-RT | GCGCAATTCAAAGCGGTTGT |
| HupB-R-RT | GGTGCCTTCTTCGCTACCTT |
a
F, forward; R, reverse.
b
Mutagenized codons are indicated in boldface. Restriction sites are underlined, and the corresponding restriction enzymes are indicated in parentheses.