TABLE 2.
Plasmids used in this study
| Plasmid | Descriptiona | Source or reference |
|---|---|---|
| pGEX-5X-3 | E. coli vector utilized to generate GST fusion proteins and to purify GST protein (GST) | GE Healthcare |
| pGEX-5X-3_hupB | Full-length HupB | This study |
| pGEX-5X-3_N-HupB | pGEX-5X-3 derivative used to purify GST-tagged N-terminal domain (1 to 108 aa) of HupB (N-HupB) | This study |
| pGEX-5X-3_N-HupB_T43A | pGEX-5X-3 derivative used to purify GST-tagged N-HupB carrying the T43A mutation (T43A) | This study |
| pGEX-5X-3_N-HupB_T45A | pGEX-5X-3 derivative used to purify GST-tagged N-HupB carrying the T45A mutation (T45A) | This study |
| pGEX-5X-3_N-HupB_T65A | pGEX-5X-3 derivative used to purify GST-tagged N-HupB carrying the T65A mutation (T65A) | This study |
| pGEX-5X-3_N-HupB_T68A | pGEX-5X-3 derivative used to purify GST-tagged N-HupB carrying the T68A mutation (T68A) | This study |
| pGEX-5X-3_N-HupB_R55E | pGEX-5X-3 derivative used to purify GST-tagged N-HupB carrying the R55E mutation (R55E) | This study |
| pGEX-5X-3_N-HupB_R55Q | pGEX-5X-3 derivative used to purify GST-tagged N-HupB carrying the R55Q mutation (R55Q) | This study |
| pGEX-5X-3_N-HupB_T74A | pGEX-5X-3 derivative used to purify GST-tagged N-HupB carrying the T74A mutation (T74A) | This study |
| pGEX-5X-3_HupB_S163A | pGEX-5X-3 derivative used to purify GST-tagged HupB carrying the S163A mutation (S163A) | This study |
| pGEX-5X-3_pknI(1-1758) | pGEX-5X-3 derivative used to purify GST-tagged PknI (PknI) | This study |
| pGEX-5X-3_pknF(1-1431) | pGEX-5X-3 derivative used to purify GST-tagged PknF (PknF) | 47 |
| pGEX-5X-3_pknG(1-2253) | pGEX-5X-3 derivative used to purify GST-tagged PknG (PknG) | 50 |
| pGEX-5X-3_pknE(1-1701) | pGEX-5X-3 derivative used to purify GST-tagged PknE (PknE) | 28 |
| pGEX-5X-3_pknH(1-1881) | pGEX-5X-3 derivative used to purify GST-tagged PknH catalytic domain1-403 (PknHcat) | 28 |
| ProExHTc_pknE_K45M(1-1701) | pGEX-5X-3 derivative used to purify GST-tagged PknE carrying the K45M mutation (PknEK45M) | This study |
| pProExHTc_pknB(1-1881) | pProExHTc derivative used to purify His6-tagged PknB (PknB) | 29 |
| pProExHTc_pknAcat(1-337) | pProExHTc derivative used to purify His6-tagged PknA catalytic domain1-337 (PknAcat) | 28 |
a
The name of purified proteins used in the study are indicated in parentheses after the vector description.