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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Oral Oncol. 2013 Nov 21;50(2):104–112. doi: 10.1016/j.oraloncology.2013.10.014

Figure 4.

Figure 4

Met knockdown in JHU-SCC-012 and JHU-SCC-019 cells. JHU-SCC-012 (A) or JHU-SCC-019 (B) cells infected with recombinant lentivirus expressing Met knockdown shRNA (1 or 2) or a non-targeting (NT) shRNA were treated without (−) or with (+) HGF and examined by Western analysis for pMet (Y1234/1235), Met, pERK, ERK, β-actin or Transferrin Receptor (TfR) levels. Representative results from triplicate experiments are shown. The relative density of pMet/Met of pERK/ERK compared to β-actin or TfR is shown beneath the respective blots. Flow cytometry detected reduced cell surface Met levels in JHU-SCC-012 (C) and JHU-SCC-019 MetKD (D) cell lines. Values are expressed as the average mode ± SEM (*** p < 0.001; ANOVA) of three independent experiments.