FIG 1.

Phenotypic and gene expression analyses of TSC1-deficient naive OT1 T cells. (A) Expression of cell surface molecules in gated CD8⁺ Va2⁺ CD44^lo CD62L^hi naive WT and TSC1 KO OT1 T cells. (B) mRNA levels of the molecules indicated in naive WT and TSC1 KO OT1 T cells. Total RNAs isolated from sorted CD8⁺ Va2⁺ CD44^lo CD62L^hi WT and TSC1 KO OT1 T cells were subjected to reverse transcription and real-time quantitative PCR analysis. The mean ± the standard error of the mean was calculated for five mice per group. The data shown are representative of two independent experiments. *, P < 0.05; **, P < 0.01 (Student t test).