FIG 4.

TSC1 deficiency impairs antigen-specific early CD8 responses. Naive WT or TSC1 KO OT1 T cells were transferred into recipient mice that were then infected with Lm-Ova as shown in Fig. 2A. Mice were euthanized on day 4 after infection. (A) Percentages and total numbers of WT and TSC1 KO OT1 T cells in the spleen. The mean ± the standard error of the mean was calculated for five mice per group. (B) Cell surface expression of the molecules indicated. (C) Percentages of IFN-γ- or TNF-α-producing, donor-derived OT1 T cells. Splenocytes were stimulated with SIINFEKL peptide of the indicated concentrations in the presence of GolgiPlug for 5 h. Bar graphs show the mean percentages of IFN-γ- or TNF-α-producing donor cells ± the standard errors. The data shown are representative of three independent experiments. *, P < 0.05; **, P < 0.01 (Student t test).