FIG 6.

Impaired survival of TSC1-deficient CD8 cells in vivo. (A) Representative fluorescence-activated cell sorter plots showing 7AAD staining within WT OT1 and TSC1 KO OT1 populations in the peripheral blood and spleen on day 6 postinfection (competitive adoptive transfers). FSC, forward scatter. (B) Percentages of 7AAD⁺ (nonviable) cells within WT OT1 and TSC1 KO OT1 populations in the peripheral blood and spleen on day 6 postinfection. The mean ± the standard error of the mean was calculated for five mice per group. The data shown are representative of two independent experiments. **, P < 0.01; ***, P < 0.001 (Student t test).