TABLE 2.
Oligonucleotide primers designed and used in this study
| Purpose | Name | Sequence (5′ to 3′)a |
|---|---|---|
| Promoter fusion construction | eps-F | GGATCCCATCGCAATATAACACAAGC |
| eps-R | ACTAGTCAGTCTCACCTCTTCTGCGC | |
| eps70-F | GATATCACTAGTAAGCTTATTACATTTGACTATTATTCA | |
| eps70-R | GATATCGGATCCGGCATCGCAATATAACACAAGC | |
| vesA-F | CTCGAGACTAGTATTCGCCAGAAAGAAGTGGG | |
| vesA-R | CTCGAGGGATCCGCGTCACCTCATTGGTTGA | |
| vesB-F | CTCGAGACTAGTTGTATCTCGGCTTACTGCT | |
| vesB-R | CTCGAGGGATCCAACTCAATCCTTTCATTAATCA | |
| vesC-F | CTCGAGACTAGTAGATACCTCTGATAACTCCC | |
| vesC-R | CTCGAGGGATCCGGCTGTAAATCTTGTATCTC | |
| tarp-F | CTCGAGACTAGTAATGCCGTACAAGGTTTT | |
| tarp-R | CTCGAGGGATCCCAGCAAATAGTTACACAATAATCA | |
| Cloning wild-type alleles | rpoE-F | ATGCATCCCGGGAGGGGAATAACAATAGGAGTGA |
| rpoE-R | ATGCATTCTAGATCATTACGGAATTTGCGTTA | |
| hapR-F | ATGCATGAATTCCAACTCAATTGGCAAGGATA | |
| hapR-R | ATGCATTCTAGAGCTGCCCAAGAAACTAGTTC | |
| Creating ΔhapR knockout | pNEB193-F | CTCTAGACTTAATTAAGGATCC |
| pNEB193-R | GACTCTAGACTTAATTAAGGATCC | |
| hapR-U-F | CCTTAATTAAGTCTAGAGTCTTCCACCAGCAATACATC | |
| hapR-U-R | CAGCCTACACTTTGCTCTAATGATTATTTTGTTATTTG | |
| kan-F | TTAGAGCAAAGTGTAGGCTGGAGCTGCT | |
| kan-R | GCCCAAGAAAATGGGAATTAGCCATGGTCC | |
| hapR-D-F | TAATTCCCATTTTCTTGGGCAGCACAAAG | |
| hapR-D-R | GCCTGCAGGTTTAAACAGTCGTCACTGCAAGATATGTTAGATG | |
| hapR-F-Sal | CGAAGTCGACTTCCACCAGCAATACATCTTTAC | |
| hapR-R-Sca | CGAAGAGCTCGTCACTGCAAGATATGTTAGATG | |
| Sequencing genomic library | pACYC-F | CCGAAGAACGTTTTCCAATG |
| pACYC-R | GAGTACTCAACCAAGTCATTCTGA |
a
F, forward; R, reverse; U, upstream; D, downstream. The underlined nucleotides refer to restriction enzyme sites.