FIG 2.

Phenotypic analysis of L. major-infected CD11b⁺ RFP⁺ cells in the ear (i.d.), footpad (s.c.), and peritoneal cavity reveals increased frequencies of infected neutrophils in the ear and preferential parasite capture by neutrophils. Samples for which results are shown in Fig. 1 were analyzed as a function of RFP expression. (A) Representative dot plots of RFP expression by CD11b⁺ cells in naïve mice or in mice injected with wild-type or RFP-expressing L. major parasites. (B) Representative dot plots and gating strategy of CD11b⁺ RFP⁺ cells. (C) Total relative number of CD11b⁺ RFP⁺ cells (left) or frequency of RFP⁺ cells within the CD11b⁺ gate (right) as a function of site. (D) Frequencies of the indicated myeloid populations among total CD11b⁺ RFP⁺ cells (filled circles) or total CD11b⁺ cells (open circles). Asterisks indicate a significant difference between total CD11b⁺ cells and CD11b⁺ RFP⁺ cells. Number signs indicate a significant difference between groups as indicated by the brackets. Horizontal lines represent means ± SD. Data are representative of 2 independent experiments.