FIG 5.

Increased neutrophil recruitment correlates with increased numbers of RFP⁺ cells following i.d. inoculation of the ear versus s.c. inoculation of the footpad. Samples for which results are shown in Fig. 3 were analyzed as a function of RFP expression. (A) Frequency of infected (RFP⁺) CD11b⁺ cells among total CD11b⁺ cells (top) or total relative number of CD11b⁺ RFP⁺ cells at each site over the indicated time p.i. (B) Representative dot plots of Ly6G and Ly6C expression on CD11b⁺ RFP⁺ cells. (C and D) Frequencies of the indicated myeloid populations among CD11b⁺ RFP⁺ cells (C) or total relative number of each population (D) at each site over the indicated time p.i. (E) Frequency of CD11c⁺ MHC II⁺ cells within the infected CD11b⁺ Ly6G⁻ Ly6C^lo RFP⁺ or CD11b⁺ Ly6G⁻ Ly6C^hi RFP⁺ population at each site over time. Each symbol represents the mean, and error bars represent SD, for 5 to 6 (ear) or 5 to 7 (f.p.) samples. Data are representative of 2 independent experiments.