FIG 6.

Intradermal inoculation of the ear results in a higher effective dose than s.c. inoculation of the footpad. Mice were infected i.d. in the ear or s.c. in the footpad with L. major-RFP. At the indicated time points, the number of CD11b⁺ RFP⁺ cells (RFP⁺ cells), or the number of parasites as determined by limiting dilution analysis (LDA), per ear or footpad was determined. Analysis was performed following needle inoculation of 1 × 10⁶ (A), 2.5 × 10⁵ (B), or 1,000 (D) metacyclic promastigotes. (C) Day 12 p.i. data from the experiment for which results are shown in panel A were plotted with the absolute number of CD11b⁺ RFP⁺ cells per site as determined by employing counting beads. Six to 12 ears or footpads were analyzed per time point. Asterisks indicate a significant difference between the ear and footpad groups (A, B, and D) or between the groups indicated by brackets (C). (D) *, P = 0.012; **, P = 0.008.