FIG 5.

RNase L regulates cytokine induction by EPEC. (A and B) Caco-2 cells stably transfected with nonspecific (shNS) or RNase L-targeted (shRNase L) shRNA were differentiated for 28 days on transwell plates, and monolayers were infected with WT or ΔescF EPEC at an MOI of 50 for 3 h. RNA was isolated and analyzed by qRT-PCR for IFN-β (A) and TNF-α (B), and GAPDH was utilized as a control. The data points shown are the means of 3 separate experiments. The error bars indicate standard deviations. P values were calculated using the Student t test. *, P < 0.05. (C) rRNA cleavage was analyzed in the indicated samples using an Agilent Bioanalyzer; RNA from cells transfected with poly(I · C) to produce endogenous 2-5A served as a positive control (+con). Arrows indicate bands which represent rRNA cleavage products. Shown is a representative image from 3 separate experiments.