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Measurement of parasite growth based on fluorescence counts of YFP-expressing parasites. Exactly 104 wild-type MonoMac6, TetR knockdown, or ALOX12 knockdown cells were infected with 5 × 103 YFP-expressing parasites in 96-well plates. Blue line, MonoMac6 cells; red line, TetR knockdown cells; green line, ALOX12 knockdown cells. Asterisks indicate a significant difference between ALOX12 knockdown cells and wild-type MM6 cells (P, <0.0482).
