FIG 5.

Analysis of the effects of ALOX12 gene knockdown on the expression of cytokines and caspase-1 in a human monocytic cell line (MonoMac6). Quantitative real-time PCR was performed on cDNA synthesized using equal amounts of total RNA from wild-type MonoMac6 cells (MM) and MonoMac6 cells engineered to express either ALOX12 gene shRNA (ALOX12-KD) or tetracycline repressor gene shRNA (TetR) that had been cultured with (shaded bars) or without (open bars) parasites for 36 h. The IL-1β (A), IL-6 (B), TNF-α (C), and caspase-1 (D) transcript levels were divided by the actin transcript level for each respective sample to yield the relative gene transcript levels. Asterisks indicate significant augmentation of gene expression attributable to infection (P, <0.05). Data are means of results from three independent experiments; error bars, standard errors.