TABLE 2.
Primers used in this study
| Primer name | Primer sequencea | Application |
|---|---|---|
| Pgn_1932F1 | GGTGGTTTGCTGTGGAAAGT | Creation of the pgn_1932 mutant |
| Pgn1932R1erm | gatgttgcaaataccgatgagcGTTGAGGTGGCCAATGAGTT | |
| Pgn1932F2erm | cctctagagtcgacctgcagTCTTCCGCTTTTGGTCACTT | |
| Pgn_1932R2 | ATCCTTGCGCATAGAACCAG | |
| 1932expF | CAACAACATATGTGGACGGCGAGCTACCTCTTC | Creation of recombinant PGN_1932 |
| 1932expR | CAACAAGGATCCTTATTTCTCTTTTTTAGCCGTTACAAATTG | |
| rgpAF160 | GTCACCGTCTGCATCGATCG | qPCR for rgp gene |
| rgpAR160 | AGTCGGCCAGAAAGTAACGC | |
| fimAF88 | CGGAACGAATAACCCAGAGA | qPCR for fimA gene |
| fimAR88 | CTGACCAACGAGAACCCACT | |
| fimR124F | TAACCACGGGTAGCCATTTC | qPCR for fimR gene |
| fimR124R | GAGTCGTTCTGCTGCTGTTG | |
| sod235F | AATTCCACCACGGTAAGCAC | qPCR for sod gene |
| sod235R | GAGCCGAATTGTTTGTCGAT | |
| Pgn0178-113F | CCACTTCTTCGGGCTTCAC | qPCR for pgn_0178 gene |
| Pgn0178-113R | CAATGGCGTATCCCTCGTAT | |
| Pgn0179-104F | TACACAAGCGAAGCTCTCCAA | qPCR for pgn_0179 gene |
| Pgn0179-104R | TGAAATTTCCAATCGTATTC | |
| Pgn0181-128F | GACGATCACATGCTGGAAGA | qPCR for pgn_0181 gene |
| Pgn0181-128F | ATTGGGCAGGTCAGTACACC | |
| Pgn0183-131F | CGGCTTCCTTGTACCCTATG | qPCR for pgn_0183 gene |
| Pgn0183-131R | CTTTCCAACGGCTACCATCT | |
| uvrAII-112F | GAAGGAACGGTGGAGGAAC | qPCR for uvrAII gene |
| uvrAII-112R | GGCATGCCCCGATAGGATTG | |
| PG16S-F | TGTAGATGACTGATGGTGAAA | qPCR for 16S rRNA gene |
| PG16S-R | ACTGTTAGCAACTACCGATGT |
a
The sequences corresponding to those of the erythromycin resistance (Emr) gene are underlined and in lowercase.