TABLE 1.

PCR primers used to construct GST-ProG-GN, GST-ProU-UGN, and GST-ProG-UGN

Primer (restriction site)	Sequence (5′–3′)	Purpose
Guanylin Fwd (EcoRI)	GCCTTGGCAGAATTCGTCACCGTGCAG	Cloning of the ProG-GN sequence into plasmid pSXG
Guanylin Rev (BamHI)	TGGGCCCATGGATCCTTAGCATCCGGT	Cloning of the ProG-GN sequence into plasmid pSXG
Uroguanylin Fwd (EcoRI)	GCAGAGCACAGAATTCGTCTACATCCAGTACC	Cloning of the ProU-UGN sequence into plasmid pSXG
Uroguanylin Rev (BamHI)	TGGGCGGATCCTACCCAGGGCTATCTCA	Cloning of the ProU-UGN sequence into plasmid pSXG
ProG-BbvCl-Uroguanylin	CGCTGAGGACAACGACGACTGTGAGCTGTGTGTGAAC	Cloning of the UGN peptide sequence into plasmid pSXG-ProG
pSXG-far-right	GGTTCCGCGCACATTTCCCCGAAAAGTG	Cloning of the UGN peptide sequence into plasmid pSXG-ProG