TABLE 3.
Transcriptional levels of Brucella genes examined using qRT-PCR
| B. abortus 9-941 ORF | Predicted protein function | 2−ΔΔCT valuea |
|---|---|---|
| BruAb1_0004 | Molybdopterin biosynthesis protein MoeB | 1.08 |
| BruAb2_1070 | Flagellar biosynthesis protein FliQ | 1.17 |
| BruAb1_1334 | Acid shock protein | 24.41 |
| BruAb1_1735 | Hypothetical protein | 1.46 |
| BruAb1_0035 | Hypothetical protein | 1.02 |
| BruAb1_1556 | Hypothetical protein | 0.82 |
| BruAb1_1726 | Hypothetical protein | 1.63 |
| BruAb1_1735 | Hypothetical protein | 1.49 |
| BruAb2_0400 | Hypothetical protein | 1.63 |
| BruAb2_0811 | Hypothetical protein | 0.34 |
| BruAb2_1055 | IS3 family transposase OrfB | 2.14 |
| BruAb1_1241 | Elongation factor G | 0.93 |
| BruAb1_0657 | Omp2b, porin | 1.26 |
| BruAb1_0822 | NADH dehydrogenase subunit G | 1.10 |
| BruAb1_1878 | Succinate dehydrogenase flavoprotein subunit | 1.21 |
| BruAb1_1239 | 30S ribosomal protein S10 | 1.25 |
| BruAb1_1255 | Elongation factor Tu | 1.00 |
| BruAb1_1238 | 50S ribosomal protein L3 | 1.28 |
| BruAb1_1242 | 30S ribosomal protein S7 | 2.04 |
| BruAb1_1243 | 30S ribosomal protein S12 | 0.90 |
| BruAb2_0505 | Glycine cleavage system protein H | 1.44 |
a
A value of 1 indicates that the gene is similarly expressed under both conditions (ΔrfbE mutant versus S2308), a value of >1 indicates that the gene is overexpressed in the ΔrfbE mutant, and a value of <1 indicates that the gene is expressed less in the mutant.