FIG 2.

Effect of mutations in Embden-Meyerhof-Parnas pathway carbon metabolism genes on S. flexneri plaque formation. Mutants with defects in EMP pathway genes were tested for the ability to invade, replicate, and spread by assessing the plaque phenotype on a Henle cell monolayer. Where indicated, the mutant strain was carrying a plasmid encoding a wild-type gene. The invasiveness of wild-type and selected mutant strains was determined and is indicated below the picture as the percentage of Henle cells containing three or more intracellular bacteria (average ± standard deviation for ≥3 assays).