TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | E. coli cloning strain; λpir | J. Kaper |
| JW0120 | gcd::Kan | Keio Collection |
| JW1666 | pykF::Kan | Keio Collection |
| JW1839 | eda::Kan | Keio Collection |
| JW1840 | edd::Kan | Keio Collection |
| JW1841 | zwf::Kan | Keio Collection |
| JW1843 | pykA::Kan | Keio Collection |
| JW3887 | pfkA::Kan | Keio Collection |
| JW5280 | pfkB::Kan | Keio Collection |
| JW3400 | gntK::Kan | Keio Collection |
| S. flexneri | ||
| 2457T | Wild-type serotype 2a | Walter Reed Army Institute of Research |
| EAWS132 | 2457T pfkA::Kan | This study |
| EAWS130 | 2457T pfkB::Kan | This study |
| EAWS118 | 2457T ΔpfkA | This study |
| EAWS122 | 2457T ΔpfkA pfkB::Kan | This study |
| EAWS105 | 2457T pykA::Kan | This study |
| EAWS107 | 2457T pykF::Kan | This study |
| EAWS120 | 2457T ΔpykF | This study |
| EAWS124 | 2457T ΔpykF pykA::Kan | This study |
| AGS200 | 2457T ppsA::Kan | 54 |
| EAWS134 | 2457T eda::Kan | This study |
| EAWS135 | 2457T edd::Kan | This study |
| EAWS144 | 2457T Δeda edd::Kan | This study |
| CFS103 | 2457T gntK::Kan | This study |
| CFS104 | 2457T gnd::Kan | This study |
| ARS100 | 2457T zwf::Kan | This study |
| ARS101 | 2457T gcd::Kan | This study |
| NHS102 | 2457T Δzwf | This study |
| NHS104 | 2457T Δzwf gcd::Kan | This study |
| Plasmids | ||
| pCP20 | Flp recombinase, Ampr, ts | 55 |
| pEda | eda cloned into SmaI site of pWKS30 | This study |
| pWKS30 | Low-copy-number cloning vector, Ampr | 56 |
| pPykF | pykF cloned into SmaI site of pWKS30 | This study |
| pWPfkA | pfkA cloned into pWSK29 | 28 |