TABLE 2.

Primers used in this study

Primer	Sequence (5′→3′)
pykA_F	ATCGCGGCGTTATTTCATTCG
pykA_R	ATCCGGCAACGTACTTACTC
pykF-F-Alex	CTCACACTGACAACTTCGGC
pykF-R-Alex	GCAGAGTAGAACCCAGGATTACC
pfkB_fwd	TTCGGTGCCAGACTGAAATC
pfkB_rev	AATGCTGGGGGAATGTTTTT
pfkA_fwd	AGCCAGACCCGCATTTTGTG
pfkA_rev	TTCATCGCCCGACTCTCTTATG
edd_fwd	TGAAACGCGTTGTGAATCAT
edd_rev	TCAGAGTTTTCTCTCGCCTGA
edd_Keio_fwd	CGCGGTGTGAATCATCCTGCTCTGACAACTCAATTTCAGGAGCCTTTATGATTCCGGGGATCCGTCGAC
eda_Keio_rev	GCCCGATCCTAGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACTGTAGGCTGGAGCTGCTTC
eda_fwd3	ACCTGCAAATCACCAAGCTG
eda_rev3	TGCCGTTATTCTGCCACAAC
gcd FWD	AAAGCTCGAATTGTGATGACG
gcd REV	AAATATGCCCGGATCTCTCC
zwf_F3	AAATTACAAGTATACCCTGGCTTAAGTA
zwf_R3	ACAACGCGTTTCATTCAGAGGATTTAT
gntK For2	ATGGAGCCACGACTTGCGAG
gntK Rev	ACTTCAAAGAACAGCGGTAGCG
gnd Keio 3′	GCCTCAATTTTATTGTTGGTTAAATCAGATTAATCCAGCCATTCAGTATGTGTAGGCTGGAGCTGCTTCG
gnd Keio 5′	TATAACGGTTAATAGACGGTTAATAATATAGAAGACAGGAGTAAACAATGATTCCGGGGATCCGTCGACC
kt	CGGCCACAGTCGATGAATCC
k2	CGGTGCCCTGAATGAACTGC