TABLE 3.

pfk, pyk, and eda mutants have reduced fitness in the plaque assay

Strain	Mutation(s)	Competitive indexa
EAWS122	pfkAB	0.379*
EAWS124	pykAF	0.043**
EAWS134	eda	0.268*

^aThe competitive index for intracellular growth of the mutants was determined by coinfection of Henle cells with equal numbers of the wild type and mutant and comparing the recovery of each strain after 72 h, as described in Materials and Methods. A competitive index of <1.0 indicates that the mutant has a disadvantage compared to the wild type. *, P ≤ 0.02, and **, P < 0.01, by t test.