FIG 1.

SEB induces lung inflammation. (A) Representative H&E images (×20) of cross sections of the lung from mice exposed to either vehicle or SEB. (B) Lung-infiltrating mononuclear cells obtained by density gradient centrifugation and total number of viable cells were counted using a hemocytometer. Cells were further stained with monoclonal antibody (MAb) to identify CD4⁺ and CD8⁺ cells and were analyzed on a flow cytometer. The percentage of the immune cell subsets was multiplied by the total number of cells found in the lung and divided by 100 to yield the absolute cell numbers shown. (C) The concentration of IFN-γ protein present in the BALF was determined using a standard ELISA kit. Data are means ± SEM (n = 5) and are representative of three independent experiments. Statistical significance compared to SEB-vehicle is indicated as follows: **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.