FIG 6.

miR-155 targets Socs1. (A) Chinese hamster ovary (CHO) cells were cotransfected with miR-155 mimic or mimic control along with plasmid containing either the 3′UTR of Socs1 or control plasmid for 24 h. Relative luciferase activity (firefly normalized to renilla) was determined following transfection. (B) Lymph nodes (LN) cells obtained from naive wild-type (WT) mice were transfected either with 40 nM miR-155 mimic (Mimic) or mimic control (Control) for 24 h. Socs1 levels were determined by RT-PCR. (C) LN cells obtained from miR-155^−/− were also transfected with 40 nM miR-155 mimic or mimic control as indicated for 24 h, and Socs1 levels were assessed. (D) LN cells were activated with SEB (1 μg/ml) for 24 h. Cells were then transfected with 100 nM miR-155 inhibitor (Inh) or inhibitor control (Inh Control) for another 24 h. Socs1 levels were determined via qRT-PCR. Data are represented as means ± SEM from replicate samples statistical significance is indicated as follows: *, P < 0.05; **, P < 0.01; ****, P < 0.0001.