FIG 4.

The pmpD null mutant is attenuated in a nonhuman primate infection model. Six cynomolgus macaques were ocularly infected with the pmpD null mutant and six were infected with the WT strain to evaluate infection, the duration of shedding, and clinical pathology. (A) Ocular infection was monitored by swabbing the conjunctiva and culturing recoverable IFU on HeLa cell monolayers. Averages and standard deviations are shown on a log₁₀ scale. Detailed chlamydial shedding data are shown in Table S3 in the supplemental material. (B) Disease was scored on the basis of hyperemia and follicle formation on the upper conjunctival surfaces (score 0 = no disease, score 12 = maximum disease). Averages and standard deviations are shown. Macaques were monitored for shedding and disease for 100 days postinfection. *, statistically significant difference (P < 0.05, two-tailed Mann-Whitney U test, n = 6).