TABLE 3.
In vitro growth favors the presence of the 350-kb duplication
| Isolate and pass | % positivea |
nb | |
|---|---|---|---|
| Junctionc | Endd | ||
| HN878-27 | |||
| 1st pass | 0 | 100 | 24 |
| 29th pass | 0 | 100 | 24 |
| 29th pass, no glycerol | 33 | 100 | 24 |
| 58797 | |||
| 1st pass | 0 | 100 | 24 |
| 29th pass | 100 | 100 | 24 |
| 29th pass, no glycerol | 4 | 100 | 24 |
a
The percentage of positive isolates is given.
b
Number of isolates tested by PCR.
c
PCR of the junction region of the duplication using primers JuntA-B and MR3128c-R3.
d
PCR of the end region of the duplication using primers JuntA-B and Rv3428c-A was used as a quality control of the template DNA.