FIG 1.

TNF-α induction in TLR2 KO macrophages. (A) Peritoneal macrophages derived from WT and TLR2 KO mice were infected with live or heat-killed M. pneumoniae. After 6 h of incubation, TNF-α concentrations in the culture medium were measured using ELISA. (B) Peritoneal macrophages derived from TLR2 KO mice were infected with heat-, sonication-, or antibiotic-killed or overgrown M. pneumoniae. After 6 h of incubation, TNF-α concentrations in the culture medium were measured using ELISA. All values are presented as the means and standard deviations (SD) of the results of 3 assays. *, P < 0.01 compared with PBS results by multiple comparison.