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. 2014 Jul;82(7):2851–2859. doi: 10.1128/IAI.01671-14

FIG 5.

FIG 5

Protease activities of feline T. foetus are attributed to serine and cysteine proteases. (A) Representative gelatin zymography of cellular protein lysates (40 μg each) from feline trichomonad isolates. Lanes 1 to 4, T. foetus isolates (A, D, F, and Sti) from four different domestic cats; lane 5, feline P. hominis. (B) The effect of inhibitors on protease activity of cellular protein lysates (40 μg each) from T. foetus (isolate A). Lanes 1 and 2, no inhibitors (vehicle-treated controls; ethanol [EtOH], diluent for pepstatin A and PMSF; dH2O, diluent for E64 and EDTA); lane 3, 5 mM PMSF; lane 4, 300 μM E64; lane 5, 10 μM pepstatin A; lane 6, 5 mM EDTA. (C) Lane 1, 40 μg of cellular protein lysate from T. foetus (isolate A); lane 2, 40 μg of secretory product from the corresponding isolate; lane 3, 275 μg of secretory product from corresponding isolate (arrow a, serine protease activity; arrow b, presumptive location of CP30/CP8; arrow c, cysteine protease activity). Results observed with isolate A are representative of assays performed concurrently with isolates D, F, and Sti.