FIG 8.
Cysteine protease activity mediates T. foetus cytopathogenicity. Spectrophotometric analysis of crystal violet absorbance of IPEC-J2 monolayers following coculture for 20 to 28 h with two different strains (F and Sti) of vehicle-treated T. foetus (dH2O) or T. foetus pretreated with E64 (300 μM). Asterisks above bars are comparisons to uninfected IPEC-J2 cells. Comparisons between vehicle-treated T. foetus and T. foetus pretreated with E64 are depicted by overhanging lines. ***, P < 0.001; **, P < 0.01; *, P < 0.05 (one-way ANOVA and post hoc Holm-Sidak test). Data represent n = 4 to 8 cultures per treatment group and are reported as means ± SD.