FIG 7.

All CoRESTs are expressed in the adult rat brain. (A) Thirty micrograms of protein extracts obtained from different nuclei of adult rat brains was fractionated on SDS-PAGE. Western blot assays were performed for the identification of CoREST1, CoREST3, LSD1/KDM1A, and β-actin (loading control) in the brain cortex (C), hippocampus (H), midbrain (M), and parabrachial nucleus (PB). (B) Thirty-micrometer-thick paraformaldehyde-fixed adult rat brain slices were incubated with rabbit anti-CoREST2 and mouse anti-CoREST1. Twenty-four hours later, slices were incubated with fluorescent secondary antibodies. Hoechst is a nuclear stain. Images were taken with a confocal microscope.