FIG 11.
Comparison of hairpin formation and efficiencies of pulldown of PCs containing 3′Dβ1 or Vβ14. Radiolabeled RSSs (32P) were incubated with protein for 1 min before the addition of biotinylated (Bio) partner and subsequent incubation for 10 min, as described in Materials and Methods. P values were calculated by a two-tailed t test with Welch's correction. *, P < 0.05; **, P < 0.01; ***, P < 0.0001. (A) The magnitude of hairpin formation at 10 min is shown for the indicated substrate pairs containing 3′Dβ1. (B) The magnitude of hairpin formation at 10 min is shown for the indicated substrate pairs containing Vβ14. (C) The efficiency of radiolabeled RSS recovery (% input) is indicated for the indicated substrate pairs containing 3′Dβ1. (D) The efficiency of radiolabeled RSS recovery (% input) is indicated for the indicated substrate pairs containing Vβ14.